FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105788
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1863225.999186035878817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG505731.6283468156873555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG131790.42433675447261693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC122120.39320133891946263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG89040.28669052749253976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG53000.17064912350746414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG38150.12283517097754258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG34960.11256402561926314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA148850.068.5841354
GGGGTTA148250.068.1309953
GGTTAAA151550.067.8471955
GGGGGTT165500.065.152352
GTTAAAA161500.064.92456
TTTGGGG282550.061.6881738
TTGGGGG285250.061.61176339
AGGGGGG344900.059.3851770
TTTTGGG298000.057.6765637
TGGGGGG314350.057.4782640
GGGGGGT200000.057.1710551
GGGGCCG30250.056.11518545
GGGCCGG30950.054.16663746
AAGGGGG377350.053.90556769
AAAGGGG377500.053.71402768
GGCCGGG30900.053.3481647
GCCGGGG31100.052.5591848
AAAAGGG388400.052.19674767
TTTTTGG330600.051.8414736
CCGGGGA20300.050.17033449