FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361839
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1570446.649225455249066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG413281.749822913416198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG111020.4700574425267768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC98660.4177253402962692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG62380.2641162246876269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG47600.20153786943140495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG40540.17164590812498226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG26510.11224304450896104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA126000.068.4892354
GGGGTTA125150.068.2818653
GGTTAAA128700.067.60480555
GGGGGTT139500.065.14194552
GTTAAAA136200.064.6786756
TTTGGGG235550.062.33757838
TTGGGGG238550.061.90052839
GGGGCCG23750.059.09421545
AGGGGGG290750.059.03802570
TGGGGGG258850.058.61451340
GGGGGGT164450.058.55930351
TTTTGGG249050.058.22767637
GGGCCGG24050.058.2090846
GGCCGGG24000.057.89286847
GCCGGGG25000.054.31952348
AAGGGGG317450.053.62800669
AAAGGGG318800.053.23202568
TTTTTGG274450.052.7893236
CCGGGGA17400.052.49174549
AAAAGGG327100.051.77317467