FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016727
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1260416.249779965260544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312351.5487966393071546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG89660.4445817406123883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC77240.38299680621125215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG46870.23240627015952087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG43350.21495224688319242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG37230.1846060473232123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA96000.068.3834653
GGGTTAA97400.068.3004254
GGTTAAA98600.067.974555
GGGGGTT105150.065.7591752
GTTAAAA106100.064.45656
TTGGGGG185800.063.51473239
TTTGGGG185000.063.45990838
TGGGGGG202050.059.87880340
TTTTGGG195250.059.2650137
GGGGGGT124600.059.03426451
GGGGCCG19850.058.0082245
AGGGGGG231500.057.89375370
GGGCCGG20000.057.57030546
GCCGGGG20200.056.13542648
GGCCGGG20700.055.6248647
AAGGGGG248150.053.76548469
TTTTTGG215550.053.62952436
CCGGGGA14050.053.29863749
AAAGGGG250900.053.19674368
AAAAGGG256950.051.88971367