FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612258
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG17413710.800814757935765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415842.5792397990892275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG121360.7527331233586684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG63800.3957183031499921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG58840.3649539961966385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG49010.3039836056015848No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG19140.11871549094499764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA132850.069.103854
GGGGTTA131250.069.0416953
GGTTAAA134050.068.8421655
GGGGGTT142450.067.39303652
GTTAAAA141050.066.6930956
TTTGGGG250200.065.6752838
TTGGGGG252700.065.34988439
TTTTGGG256000.063.39420337
TGGGGGG272150.062.36235440
GGGGGGT167200.061.12399351
GGGGCCG27200.060.59868245
GGCCGGG27050.060.1603547
AGGGGGG311350.060.0097570
CCGGGGA17450.059.56795549
GGGCCGG27500.059.42858546
TTTTTGG272950.059.24852836
GCCGGGG27150.058.40102848
GGGCCCC6150.056.897829
GGGTTTA9550.055.70643654
GGCCCCC6100.055.0764710