FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704451
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG18164410.657038542029076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG419682.4622591086514074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG125870.7384782548750302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC109790.6441370271131291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG74470.4369148775764161No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG64880.3806504264422973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG46550.27310846718386156No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21310.12502559475162384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA140250.069.3281853
GGGTTAA141600.069.1348354
GGTTAAA142600.068.7542555
GGGGGTT153050.067.7379652
GTTAAAA150850.066.4559256
TTGGGGG264950.065.98057639
TTTGGGG263700.065.9235938
TTTTGGG271100.063.1261537
TGGGGGG286750.062.37014440
GGGCCGG26350.060.81877546
GGGGGGT182700.060.67044451
GGGGCCG26850.060.4752345
GGCCGGG26150.060.21877347
CCGGGGA17550.059.03129249
TTTTTGG289600.058.91049636
AGGGGGG336650.058.7768270
GCCGGGG26750.058.7441648
GGTTTAA11700.056.8425755
AAGGGGG365400.053.9286769
AAAGGGG365250.053.86764568