FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215889
Sequences flagged as poor quality0
Sequence length76
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG11083451.33841928027829No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2525411.697677973403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG75273.486513902977919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC66003.0571265789363977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG44822.076066867695899No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG39911.8486351782629036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG28891.338187679779887No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG13070.6054037028287684No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC12460.5771484420234473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG8180.37889841538938995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG7500.3474007476064089No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG7210.33396791869896103No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG7160.3316519137149183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGGGGGGGGGG6390.2959854369606603No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG6140.2844054120404467No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC5900.2732885881170416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTATTTGGGGGGGGGG5780.26773017615533906No Hit
GGGGGGGAAAAAAGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC5280.24457012631491182No Hit
GGGGGGGGGGGCCCCCCGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC4850.2246524834521444No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC4190.19408121766278041No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4030.18667000171384368No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3930.18203799174575824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTATTTTTGGGGGGGGGG3820.17694278078086423No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3330.15424593193724553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGCC3010.1394235000393721No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG2960.13710749505532935No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC2940.13618109306171225No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2720.12599067113192428Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGTGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2630.12182186216064736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAAAATTTTTTTTTGGGGGGGGGG2490.11533704820532774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAATATTTTTTTTTGGGGGGGGGG2470.11441064621171065No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC2470.11441064621171065No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2380.11024183724043374No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG2320.10746263125958247No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2270.10514662627553975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTT150.002221482370.0076652
TGGTCGC301.01956175E-770.0076642
GCGATTA150.002221482370.0076653
AGAAGGA150.002221482370.0076653
GGCGATT150.002221482370.0076652
AGAATTT150.002221482370.0076628
GGGAGTA150.002221482370.0076652
GAGTTAA1250.070.0076654
GGCCCCC3050.070.0076610
ATTGCGG150.002221482370.0076638
GCGTTAA150.002221482370.0076654
GGTGGTC301.01956175E-770.0076640
GTGGTCG301.01956175E-770.0076641
CGGTTAA301.01956175E-770.0076654
GGAGTAA150.002221482370.0076653
AAGTTGT150.002221482370.0076629
GGTCGCC301.01956175E-770.0076643
CGATTAA150.002221482370.0076654
AAATTGT207.90975E-570.0076529
CCGCCGG207.90975E-570.0076512
Produced by FastQC (version 0.11.5)