FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241416
Sequences flagged as poor quality0
Sequence length76
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG12035749.854607813898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2773711.489296484077277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG84983.5200649501275807No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC70182.90701527653511No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG57252.371425257646552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG45991.9050104384133613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29871.2372833615004806No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG15740.6519866123206416No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC13570.5621002750439076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG11280.46724326473804556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG9290.3848129370050038No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG8700.3603737946117904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTATTTGGGGGGGGGG8460.35043244855353417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGGGGGGGGGG8180.33883421148556847No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG7930.32847864267488486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG7890.32682175166517546No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC6770.2804288033933128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTATTTTTGGGGGGGGGG5640.23362163236902278No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC5610.23237896411174072No Hit
GGGGGGGAAAAAAGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC5550.22989362759717663No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGAAAAAATTTTTTTTTGGGGGGGGGG5440.22533717732047587No Hit
GGGGGGGGGGGCCCCCCGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC5070.21001093548066407No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4350.1801868973058952No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG4010.16610332372336548No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4000.1656891009709381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAATATTTTTTTTTGGGGGGGGGG3620.149948636378699No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3590.14870596812141698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGCC3310.1371077310534513No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC2930.1213672664612122No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2860.11846770719422077No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2670.1105974748981012No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG2630.10894058388839181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTGGGGGGGGGGGGGGGGGG2540.1052125791165457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAAAATTTTTTTTTGGGGGGGGGG2510.10396991085926369No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGAAAAAATTTTTTTTTGGGGGGGGGG2450.10148457434469961No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2430.10065612883984493Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC353.6798156E-970.0412933
CGTGGAG150.002217574370.0412815
AAATTGT500.070.0267629
AATTTGT207.9009646E-570.0267630
CAATTTT150.002219400170.0267629
AAGATTT301.0181611E-770.0267628
GATTTTT252.8277573E-670.0267630
AGTTGTT150.002219400170.0267630
AGTTGGT150.002219400170.0267630
CCCCCCG800.070.0267612
AAAGTTG252.8277573E-670.0267628
AGATTTT252.8277573E-670.0267629
AAAGATT207.9009646E-570.0267627
AATTGTT500.070.0267630
AAGTTGT150.002219400170.0267629
TTAAGGG353.6925485E-970.0122437
ATATGGG1500.070.0122437
TATAGGG353.6925485E-970.0122437
TTTTGGC150.00222122770.0122337
TGGTCGC301.0196163E-770.0122342
Produced by FastQC (version 0.11.5)