FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986921
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1199576.037331126904391No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300051.5101254654815164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG79470.3999655748769076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC72480.36478551487452193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG57920.2915063054847173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG46190.2324702391287827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29350.147715988708157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA92750.068.72089454
GGGGTTA93000.068.3103453
GGTTAAA94450.067.8631355
GGGGGTT101350.066.5125252
TTGGGGG177850.064.9313339
TTTGGGG178100.064.50114438
GTTAAAA103450.063.8541256
TGGGGGG196100.061.0834440
GGGGGGT119700.060.38203451
TTTTGGG187900.059.92473637
AGGGGGG226400.057.95522370
GGGCCGG21350.056.3980746
GGCCGGG21050.055.54320547
GGGGCCG21700.055.48982645
TTTTTGG207650.054.10581636
AAGGGGG244550.053.40650669
AAAGGGG244750.053.25515768
GCCGGGG22250.052.38764648
AAAAGGG249750.052.2143967
AAAAAGG261400.050.0333766