FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886279
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG22100211.716294355182875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG513472.7221317737195823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG139060.7372186193028709No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC131770.6985711021540292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG104760.5553791353240957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG85160.45147085876479565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG53130.2816656496732456No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG27620.14642584686570756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG18870.10003822340173432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA170100.069.1073454
GGGGTTA168500.068.9743153
GGTTAAA171550.068.87733555
GGGGGTT181200.067.7709652
GTTAAAA179900.067.1395456
TTTGGGG319300.066.2081538
TTGGGGG321500.066.0799939
TTTTGGG323000.064.3967437
TGGGGGG353700.062.0320640
GGGCCGG36200.061.48142646
TTTTTGG339650.061.02572336
GCCGGGG35650.060.96563748
GGGGCCG37350.060.80965845
GGGGGGT215000.060.80843451
GGCCGGG36150.060.79849647
CCGGGGA24800.060.25361649
AGGGGGG407000.058.87489370
TATGGGG10350.055.80702638
GGGCCCC7800.055.6346749
TTTTTTG375450.054.90700535