FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478929
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1827317.371368845174669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434621.7532571525848462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG126510.5103413611281323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC104880.4230859375157578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG92480.3730643354448635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG72630.2929894321297625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG41270.16648318689240393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA141400.069.0075354
GGGGTTA141500.068.4132353
GGTTAAA143950.068.1865155
GGGGGTT153300.066.6146852
GTTAAAA154500.065.5455656
TTTGGGG266550.065.1367938
TTGGGGG268850.065.0898239
TGGGGGG297600.061.35373740
TTTTGGG278150.061.26251637
GGGGGGT183100.059.7079251
GGGCCGG30000.058.6801346
GGCCGGG29750.057.88387347
GGGGCCG30950.057.78710645
AGGGGGG356150.057.05370370
TTTTTGG301950.056.2900836
GCCGGGG30250.055.8857648
AAGGGGG375400.053.90319469
CCGGGGA21100.053.7400649
AAAGGGG376250.053.68947668
AAAAGGG383050.052.6896167