FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437892
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1326879.227883596264531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333202.3172811309889756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG89860.6249426243417447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC82570.5742434063198071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG43690.30384757686947284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG42000.2920942602086944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG41510.288686493839593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG16460.11447313150083595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA103250.069.1783953
GGGTTAA104850.069.1240554
GGTTAAA106700.068.5159255
GTTAAAA111900.066.6165556
GGGGGTT114750.066.0858652
TTTGGGG194700.064.1928638
TTGGGGG196100.063.91082839
TTTTGGG202650.060.86495237
TGGGGGG214550.060.14400540
AGGGGGG238150.059.8333970
GGGGGGT136200.059.04393451
GGGCCGG19500.058.50601246
CCGGGGA13600.058.40621649
GGGGCCG20250.057.20321745
GGCCGGG20000.057.04137447
TTTTTGG216900.056.74930636
GCCGGGG20300.055.1659948
AAGGGGG259150.054.60476369
AAAGGGG258650.054.59423468
GGTTTAA9000.053.6604255