FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520843
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG17459011.47981744335214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG427942.8138341696019906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG115090.7567513543475559No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC101120.664894403958857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG82540.5427253174719547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG65640.4316027361141157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG42770.28122560974406957No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21890.14393333171142583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA137000.069.2782254
GGGGTTA136100.069.0677853
GGTTAAA138900.068.79088655
GGGGGTT147550.067.5504752
GTTAAAA146300.067.1796356
TTTGGGG252700.065.8028538
TTGGGGG255850.065.4219139
TTTTGGG259500.063.22865337
TGGGGGG281500.061.2989940
GGGGCCG25950.060.9617345
GGGGGGT177450.060.87989451
TTTTTGG270550.060.46805636
GGGCCGG25900.060.13151646
GGCCGGG25300.060.0359147
CCGGGGA17000.059.2905549
GCCGGGG25450.058.85890648
AGGGGGG326550.058.2727970
GGTTTAA10150.056.89687355
GGGTTTA10450.054.58821554
AAAGGGG349100.054.1201468