FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259924
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG27846312.321786042362486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG671422.97098486497776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC169200.7486977438179337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG166630.7373256799786188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG132380.5857719109138184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG112980.4999283161734642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG61580.27248703938716523No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG35890.15881065026965507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG27700.12257049352102106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA214400.069.5603154
GGGGTTA213600.069.1490153
GGTTAAA217600.068.8575455
GGGGGTT232000.067.48015652
GTTAAAA227300.067.4434656
TTTGGGG402950.066.3993138
TTGGGGG407950.066.0603239
TTTTGGG407900.064.4179237
TGGGGGG448500.062.0075740
GGCCGGG45200.061.31751347
CCGGGGA31350.061.17981349
TTTTTGG429200.061.0620736
GGGCCGG45900.060.8398346
GGGGGGT275550.060.5721351
GGGGCCG47000.060.38786345
GCCGGGG45900.059.85799448
AGGGGGG518550.058.3074570
GGGCCCC10550.056.057289
CGGGGAA34850.055.3343450
TATGGGG12750.055.1797738