FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301124
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2378237.204303746239161No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG582501.7645504985574612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG162380.4918930643017348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC147790.44769599687863887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG78940.23913067185601025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG75510.22874027149540582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG74410.22540807312903122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA182000.068.7730654
GGGGTTA182200.068.15967653
GGTTAAA185250.067.9066255
GGGGGTT200200.066.3283452
GTTAAAA197100.065.12031656
TTGGGGG346600.064.09132439
TTTGGGG344250.064.06505638
TGGGGGG375250.060.51218440
TTTTGGG360650.060.42574337
AGGGGGG424800.059.43786670
GGGGGGT238600.059.20339251
GGCCGGG37650.058.47514347
GGGGCCG38650.058.0462845
GGGCCGG38450.057.9832346
CCGGGGA26800.056.80769749
GCCGGGG38400.056.3321248
TTTTTGG395600.054.98332236
AAGGGGG463150.054.16447469
AAAGGGG465200.053.7702368
AAAAGGG477550.052.32103367