FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911016
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2106095.385020158444762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG514491.3154893766734783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG130330.33323821738392273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC124010.3170787335055648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG103450.26450927329369145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG82750.21158184983134817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG48220.12329277098329437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA166550.067.7690154
GGGGTTA164950.067.74914653
GGTTAAA169100.067.1256355
GGGGGTT180850.065.50510452
TTGGGGG315950.063.86790539
TTTGGGG313250.063.8294538
GTTAAAA183900.063.7806456
TGGGGGG347100.060.30430640
TTTTGGG331500.059.2489637
GGGGGGT215500.059.08210851
AGGGGGG396700.057.62831570
GGGGCCG35450.057.26672745
GGGCCGG35450.056.57412746
GGCCGGG35700.054.90410647
GCCGGGG35750.054.43918248
TTTTTGG364700.053.71198736
AAGGGGG426100.053.4542469
AAAGGGG427250.053.12804868
AAAAGGG439000.051.69543567
CCGGGGA27700.049.2761249