FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161072
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG19553716.841074455330933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478364.119985668416773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC129191.1126786280265133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG118561.0211253048906528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG79710.6865207325643887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG59480.5122851985062081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG45190.3892092824562129No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG31380.2702674769523337No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG17090.1471915609023385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA152000.069.49406453
GGGTTAA153600.069.3854154
GGTTAAA155450.069.0669255
GTTAAAA159950.068.23997556
GGGGGTT166450.067.9971652
TTTGGGG284050.065.28030438
TTGGGGG288850.064.6812339
CCGGGGA18800.064.593349
GGGCCGG30600.064.3960346
TTTTGGG286100.064.0634137
GGCCGGG31200.062.93599347
GGGCCCC5850.062.7994239
TTTTTGG292250.062.58470536
GGGGCCG31800.062.40623545
GGCCCCC5950.061.16384510
GCCGGGG32150.060.8664348
AGGGGGG344200.060.65600270
GGGGGGT197150.060.6421251
TGGGGGG314850.060.54049740
GCCCCCG4600.060.10147511