FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400351
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1303479.308166309732345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316842.262575597118151No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG88660.6331269803070801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC75450.5387934882040288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG54970.39254444064381006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG48110.34355672256455705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG33820.24151087834407228No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG16460.11754195912310557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAA100700.068.9586755
GGGTTAA100300.068.9520454
GGGGTTA99250.068.8023853
GTTAAAA107450.066.64628656
GGGGGTT109650.066.32580652
TTTGGGG191250.065.0624538
TTGGGGG193650.064.7552539
TTTTGGG196750.062.2118537
CCGGGGA13450.061.6740949
TGGGGGG211800.061.08767340
GGGCCGG20900.060.11651246
GGGGCCG21400.059.0390145
GGGGGGT132700.058.84863751
AGGGGGG246050.058.31258870
GGCCGGG21200.058.27737847
TTTTTGG211250.057.75116336
GCCGGGG21300.057.1883348
GGTTTAA7850.056.17946655
GGGTTTA7850.056.1774654
AAAGGGG263050.054.1847768