FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651192
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG992116.0084472308489865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247481.498796021298553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG71170.4310219526257395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC58460.35404725798090103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG39630.24000843027340246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG33670.20391329415355694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG27360.16569847722130437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA76650.068.0864154
GGGGTTA76550.067.62871653
GGTTAAA78500.066.8466455
GGGGGTT84300.065.2294352
TTTGGGG145950.064.08459538
TTGGGGG147550.063.78330639
GTTAAAA84850.063.4942556
TGGGGGG161700.059.89003440
TTTTGGG154450.059.56236637
GGGGGGT100600.058.6964851
AGGGGGG180800.058.6896570
GGGGCCG14750.056.47807745
GGGCCGG14850.055.62634346
GGCCGGG14350.055.36760747
CCGGGGA9850.054.36390349
GCCGGGG14550.054.1287148
AAGGGGG196850.053.5115669
AAAGGGG197400.053.2286768
TTTTTGG174850.052.380236
AAAAGGG204250.051.3048267