FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365274
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1387495.866085705081103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340031.4375924311517396No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG82010.346725157423622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG73110.30909738152958177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG58620.24783598010209387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG30910.13068253403199798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA106950.068.5606453
GGGTTAA108600.068.2602254
GGTTAAA110650.067.5016855
GGGGGTT118750.065.2257952
TTGGGGG210100.064.7959539
GTTAAAA120750.064.20206556
TTTGGGG211350.063.90198538
TGGGGGG232650.060.66713340
TTTTGGG222300.059.7149737
GGGGGGT142400.058.4226951
AGGGGGG264400.057.79670370
GCCGGGG24100.056.350248
GGCCGGG24450.056.2569447
GGGCCGG25100.055.63201546
GGGGCCG25450.055.55566845
TTTTTGG245900.053.94170836
CCGGGGA18650.053.4856949
AAGGGGG288850.052.63897769
AAAGGGG289400.052.40601368
AAAAGGG298750.050.76585867