FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881465
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1753969.322310008424287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG432692.299750460412498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG115480.6137770301334332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC107830.573117225141047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG58360.3101838195236159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG57450.30534716298203796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG49140.2611794532452105No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG19040.10119773686993912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA137200.068.9536954
GGGGTTA136300.068.69182653
GGTTAAA139600.068.19626655
GGGGGTT150700.066.539152
GTTAAAA147300.066.03329556
TTTGGGG254150.065.2537138
TTGGGGG257550.064.83903539
TTTTGGG261250.062.56756637
TGGGGGG277650.061.45455640
GGGGCCG26800.059.67931745
AGGGGGG313700.059.64264770
GGGGGGT178800.059.36730651
GGGCCGG26850.059.04522346
CCGGGGA17300.058.4600949
TTTTTGG280600.058.15470536
GGCCGGG26850.057.61452547
GCCGGGG26900.056.7267948
AAGGGGG340150.054.66530669
AAAGGGG340550.054.48502768
AAAAGGG345400.053.7763667