FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169389
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2096029.6617987829753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG517532.3856025821095246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC132090.6088811181397158No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG129880.5986939179649201No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG88660.4086865011300417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG77070.3552613201228549No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG53500.24661321690116433No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG25080.11560858840899443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA160600.069.0837554
GGGGTTA159450.068.8372953
GGTTAAA162350.068.5530855
GGGGGTT174250.066.78532452
GTTAAAA172350.066.3797756
TTTGGGG305700.065.0850138
TTGGGGG308200.064.9500939
TTTTGGG313000.062.52554337
GGCCGGG32650.061.9650747
GGGCCGG34000.061.15046746
TGGGGGG340200.060.48690840
GGGGCCG34900.060.3772545
GCCGGGG33300.060.332348
GGGGGGT209500.059.92797551
AGGGGGG378900.059.39106470
TTTTTGG334850.058.4661236
CCGGGGA23200.058.3855349
GGGCCCC8200.055.900979
GGTTTAA12900.053.9902755
AAAGGGG413950.053.9228668