FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038106
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1867229.161545081561018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG446352.1900234825862834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG127180.6240107236816927No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC111950.5492844827501612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG75860.3722083149747854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG68340.335311313543064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG49490.24282348415636873No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG22210.1089737236434219No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA143600.068.8687554
GGGGTTA142150.068.7342353
GGTTAAA145150.068.4069455
GGGGGTT154900.066.823652
GTTAAAA154100.066.0708156
TTGGGGG271850.065.154339
TTTGGGG270550.064.86883538
TTTTGGG278700.061.9292637
TGGGGGG296900.061.39022440
GGGGGGT183550.060.550851
GGGCCGG28700.060.2469346
GGGGCCG28900.060.19629745
GCCGGGG28450.058.56757448
AGGGGGG344750.058.2683270
GGCCGGG28850.058.2351647
TTTTTGG298900.057.64501636
CCGGGGA19400.057.18937349
GGGCCCC6250.054.3000379
AAAGGGG372100.053.75809568
AAGGGGG372750.053.7491869