FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379003
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG24296710.212975771783391No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG592172.4891519682825116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG151450.6366112190695009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC144180.6060521991775546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG118090.49638440977165643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG97970.4118111662742754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG56270.23652765465196976No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG32180.1352667482975011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG23820.10012597714252568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA190500.069.1927954
GGGGTTA189400.068.9804553
GGTTAAA193100.068.6555655
GGGGGTT205750.067.27090552
GTTAAAA203950.067.1324256
TTTGGGG356300.065.75310538
TTGGGGG359850.065.4672339
TTTTGGG362100.063.60603737
TGGGGGG395550.061.40813440
GGGCCGG39500.060.4237846
GGGGGGT246550.060.4111751
GGGGCCG40450.059.9601645
GGCCGGG39250.059.8278647
TTTTTGG385700.059.6549436
GCCGGGG39100.059.2580848
CCGGGGA26650.058.8315949
AGGGGGG454050.058.69030870
AAGGGGG489750.054.27747769
AAAGGGG490050.054.1571168
AAAAGGG496850.053.39476867