FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604315
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1572289.800319762640129No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG381892.380392877957259No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG107920.6726858503473445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC94810.5909687312030368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG58410.3640806200777279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG53710.3347846277071523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG45560.283984130298601No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG18350.11437903404256644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA119000.069.30904454
GGTTAAA120800.068.725855
GGGGTTA117900.068.7091353
GGGGGTT130100.066.8632552
GTTAAAA127200.066.3960656
TTTGGGG228000.065.00027538
TTGGGGG229600.064.9396339
TTTTGGG234550.062.18523437
TGGGGGG250150.061.03195640
GGGCCGG24800.060.96260546
GGGGCCG25550.060.1319245
GGCCGGG24750.060.09594747
GCCGGGG24600.059.472148
GGGGGGT156450.059.251251
CCGGGGA15900.058.7649649
AGGGGGG288800.058.60743770
TTTTTGG249900.058.1883736
GGGTTTA11350.054.2617854
AAGGGGG311850.053.8759269
AAAGGGG313550.053.491768