FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468179
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG662134.509872433810864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173671.1828939114372292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG42180.2872946691105104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC38990.2655670732247226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG32500.22136265400880956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG25560.1740932134296976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG15530.10577729282328653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA52400.067.8579754
GGGGTTA52800.067.0810453
GGTTAAA53500.066.7312555
GGGGGTT57950.064.436952
TTGGGGG103050.062.6955839
GTTAAAA58700.062.48935356
TTTGGGG103450.062.01758638
TGGGGGG111400.060.0677240
GGGGGGT69650.058.68946551
AGGGGGG128600.057.5641770
TTTTGGG111600.056.26344337
GGGCCGG10350.054.1062846
GGGGCCG10600.053.49421745
GGCCGGG10600.053.1621947
AAGGGGG138800.053.13221769
AAAGGGG140500.052.41996468
AAAAGGG145400.050.62242567
GCCGGGG11050.050.04695548
TTTTTGG129650.048.50972436
AAAAAGG154050.047.94061766