FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748969
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1232844.484735913718925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302171.0992121046108558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG80800.2939283782392599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC73660.2679550042215827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG53650.19516407787792442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG45260.164643544543427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG28710.10443915518872711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA96600.067.67834554
GGGGTTA95700.067.6187353
GGTTAAA98250.066.7996455
GGGGGTT106450.064.8962852
TTGGGGG185800.063.4807539
TTTGGGG186650.062.708738
GTTAAAA107450.062.25290356
TGGGGGG200350.060.73756840
GGGGGGT124250.059.85775451
AGGGGGG228350.058.4831270
GGGGCCG20400.057.3057145
TTTTGGG202500.057.1425237
GGGCCGG21250.055.34189646
GGCCGGG20600.053.86090547
GCCGGGG20550.053.65619748
AAGGGGG252500.052.7796469
AAAGGGG255100.052.1759468
AAAAGGG264900.050.13722667
TTTTTGG231400.049.87942536
GGGGGCC24000.048.56224444