FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783571
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1003225.624783089655528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257941.4461997868321474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG66740.37419312155221185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC59720.3348338810173523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG41660.23357634767553406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG37250.20885067092927617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG25050.14044857199405014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA80200.067.8206254
GGGGTTA79650.067.8055553
GGTTAAA81450.067.267655
GGGGGTT88400.064.89690452
TTTGGGG152850.063.85004438
TTGGGGG153450.063.77393339
GTTAAAA88150.063.66220556
TGGGGGG167150.059.991740
TTTTGGG164200.058.60346637
AGGGGGG187850.058.42474770
GGGGGGT107200.057.5923151
GGGGCCG17400.055.71589345
GGCCGGG17250.054.78322647
GGGCCGG17750.054.21987546
AAGGGGG203750.053.5890569
AAAGGGG206000.052.94170468
GCCGGGG17800.052.69870448
TTTTTGG185050.052.1590136
AAAAGGG211900.051.41950667
GGGGGCC19250.049.9991644