FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281737
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1115334.888074304794987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279971.2270038133229202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG76240.3341314095358054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC67240.29468777514674127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG39320.17232485601977793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG38920.17057180560248617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG30150.13213617520336482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA86200.067.7663354
GGGGTTA85950.067.3496753
GGTTAAA87350.067.07744655
GGGGGTT96950.063.92613652
GTTAAAA94600.063.49209256
TTGGGGG165350.063.35303539
TTTGGGG166600.062.7612938
TGGGGGG180850.059.7811640
AGGGGGG204300.057.97569770
TTTTGGG180350.057.39530637
GGGGGGT115500.057.11578451
GGGGCCG18900.053.51930645
AAGGGGG220850.053.34585669
AAAGGGG222750.052.741868
GGGCCGG19800.051.6169246
AAAAGGG230050.051.01067767
GCCGGGG19500.050.4410748
GGCCGGG19600.050.35899447
TTTTTGG206300.050.07874336
AAAAAGG243550.048.326966