FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690162
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1168986.9163784299966515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279251.652208486523777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG81500.48220229776790624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC68310.4041624412334439No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG53310.3154135520737066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG44650.2641758600654848No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29550.17483531164468258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA88400.068.8831254
GGGGTTA87800.068.594553
GGTTAAA91250.067.088855
GGGGGTT96900.066.0852552
TTGGGGG171150.064.39661439
GTTAAAA97750.064.3106156
TTTGGGG171350.064.0430838
TGGGGGG185500.061.22449540
TTTTGGG177500.060.6426937
GGGGGGT117850.058.67423251
AGGGGGG218300.057.43685570
GGCCGGG19350.056.6148947
GGGCCGG19900.056.45551746
GGGGCCG20250.056.34725645
CCGGGGA13300.055.78111349
GCCGGGG19450.055.42571648
TTTTTGG195550.054.96538536
AAAGGGG233400.053.4123268
AAGGGGG233900.053.36664669
AAAAGGG238750.052.1583367