FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431792
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1584146.514290695914782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383461.5768618368676268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG109110.4486814661780284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC94450.3883967049813471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG53770.22111266095126555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG52810.21716495489745835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG44990.1850075993341536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA121400.068.6436654
GGGGTTA120450.068.3424153
GGTTAAA123800.067.7439755
GGGGGTT133700.065.7813752
TTGGGGG236350.064.7079639
GTTAAAA132600.064.5152156
TTTGGGG236150.064.3167438
TGGGGGG254600.061.2370440
TTTTGGG251250.059.48757637
GGGGGGT158550.059.0275751
AGGGGGG290250.058.7313770
GGGCCGG26300.055.4920946
GGGGCCG26550.055.36617345
TTTTTGG274050.054.47722636
GGCCGGG26050.054.41130447
AAGGGGG314250.053.9184569
GCCGGGG26050.053.8750248
AAAGGGG315450.053.6456268
AAAAGGG323000.052.35915867
CCGGGGA16650.052.1279249