FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745812
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1586405.7775259194730015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382721.3938317699827956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG105400.3838573070552536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG55320.20147045755499648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG51150.1862836931297554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG44100.16060822809427594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA123250.068.3502954
GGGGTTA122800.068.0034953
GGTTAAA125650.067.3560655
GGGGGTT134700.065.6569852
GTTAAAA135200.063.91860656
TTGGGGG235250.063.4547839
TTTGGGG233600.063.35323738
TGGGGGG251850.060.80104440
GGGGGGT158200.059.53251
AGGGGGG283100.059.19449270
TTTTGGG249200.058.55643537
GGGGCCG25150.056.22150445
GGGCCGG25700.054.6077846
GCCGGGG25000.054.0438248
AAGGGGG310350.053.6924869
GGCCGGG25500.053.668547
AAAGGGG311900.053.3875868
TTTTTGG276950.052.74850536
AAAAGGG319400.052.07631367
AAAAAGG338300.049.2600566