FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349584
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1307435.564516952788239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336861.4337006040218183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG89090.37917350475658673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG42040.1789252906046347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG40940.1742436107838664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG40430.1720730137760557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA101750.068.30765554
GGGGTTA101350.067.85210453
GGTTAAA103700.067.5732355
GGGGGTT111300.065.4965852
GTTAAAA112050.063.91074456
TTGGGGG194550.063.0548339
TTTGGGG193850.062.82112538
TGGGGGG208300.060.30394740
GGGGGGT131650.058.93572251
AGGGGGG236250.058.6263570
TTTTGGG208550.057.73725537
GCCGGGG20350.056.5851348
GGCCGGG21000.055.6670247
GGGGCCG21450.055.48412345
GGGCCGG21800.054.2675646
AAGGGGG256950.053.5605469
AAAGGGG257950.053.25116368
TTTTTGG233550.051.62959736
AAAAGGG267150.051.4293367
AAAAAGG280700.048.99869566
Produced by FastQC (version 0.11.5)