FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404323
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1036414.310610512813795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255301.0618373654454913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG72980.3035365880541009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG44460.18491691840073068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG36300.1509780507860217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG26320.10946948475724769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA78000.067.7501853
GGGTTAA78800.067.7313654
GGTTAAA80250.066.7706255
GGGGGTT86600.064.57835452
TTGGGGG155400.062.86374339
TTTGGGG156250.062.3266538
GTTAAAA89950.061.1253356
TGGGGGG168700.059.34052740
AGGGGGG188150.058.84185870
GGGGGGT104900.057.28262351
TTTTGGG171000.056.25450537
GGGGCCG18150.054.37629745
AAGGGGG206600.053.07774769
AAAGGGG209100.052.2018568
GGGCCGG19000.051.01949746
AAAAGGG218600.049.885267
GGCCGGG19600.049.27810347
GCCGGGG19450.049.12553848
TTTTTGG196800.048.79836336
AAAAAGG234300.046.72178666