FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797960
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1102316.130892789605999No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285161.586019711228281No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG71350.3968386393468153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC66830.3716990366860219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG39030.2170793566041514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG38900.21635631493470375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG32880.18287392378028433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA84150.068.7457753
GGGTTAA85150.068.43162554
GGTTAAA86950.067.5841255
GGGGGTT94300.064.9090752
GTTAAAA93600.064.2425856
TTTGGGG164100.063.13334338
TTGGGGG165150.063.08696739
TGGGGGG181200.059.06342740
TTTTGGG173300.058.93351737
AGGGGGG200950.058.45174470
GGGGGGT114300.057.25462351
GGGGCCG19000.053.97763445
GGGCCGG18700.053.5318446
AAGGGGG219400.053.16797669
AAAGGGG218950.053.1378368
TTTTTGG192650.052.91890736
CCGGGGA13050.052.56504449
AAAAGGG224300.051.8362967
GCCGGGG18700.051.66153748
GGCCGGG19000.051.398547