FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409115
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1162264.824427227425839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307231.2752815868067735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG84930.35253609728053664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG52770.21904309258794205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG32260.13390809488131533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG24370.10115747899124782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA89700.067.9264754
GGGGTTA89800.067.2648453
GGTTAAA91500.066.9017655
GGGGGTT99850.064.48881552
GTTAAAA99150.062.79890456
TTGGGGG170250.061.71780439
TTTGGGG169900.061.13025338
AGGGGGG206050.058.58418370
TGGGGGG183600.058.27746240
GGGGGGT119050.057.2618251
TTTTGGG188400.054.6433337
GGGCCGG18400.054.59021846
GGGGCCG18800.053.42983645
AAGGGGG226100.052.9866169
AAAGGGG226200.052.69891768
GCCGGGG19200.051.22617748
AAAAGGG233400.051.04112267
GGCCGGG19500.050.9733947
CCGGGGA13950.047.91724449
TTTTTGG214350.047.8577336