FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052023
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG11860211.273707894219044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273442.5991827174881155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG81130.771180858213176No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG51970.4940006064506194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG42290.40198740902052515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG30160.286685747364839No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG14160.13459781772831964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA92550.069.1543854
GGGGTTA91900.069.00275453
GGTTAAA94350.068.32046555
GGGGGTT99050.067.73160652
GTTAAAA98500.067.0439256
TTTGGGG169650.065.7940238
TTGGGGG171350.065.5937839
TTTTGGG173250.063.45713837
GGCCGGG17300.062.10644547
TGGGGGG187700.061.5025140
GGGCCGG17900.060.99936746
GCCGGGG17350.060.9130848
GGGGGGT118000.060.67736451
TTTTTGG181550.060.4924436
CCGGGGA11250.060.34365549
GGGCCCC3150.059.988179
AGGGGGG215900.058.91393370
GGGGCCG18800.058.64042745
GGTTTAA7000.054.9917755
TTTTTTG199200.054.6529735