FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463678
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1500306.089675680019873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369361.4992218950690797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG96280.39079782341685887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC88780.3603555334747479No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG67330.2732905842403106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG55890.22685594464861075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG36230.14705655528035724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA116250.068.4887854
GGGGTTA115550.068.29793553
GGTTAAA118000.067.68616555
GGGGGTT126350.065.8906452
GTTAAAA127850.064.4971956
TTGGGGG221200.064.4327439
TTTGGGG220100.064.4221138
TGGGGGG239950.061.16289540
TTTTGGG232500.059.84698537
GGGGGGT151850.058.5821451
AGGGGGG276150.058.03761370
GCCGGGG24500.056.9974548
GGGGCCG25650.056.76505345
GGCCGGG24800.056.73133547
GGGCCGG25250.056.5519346
CCGGGGA16850.054.4111949
TTTTTGG257250.054.07000736
AAGGGGG296850.053.75033669
AAAGGGG297600.053.435468
AAAAGGG305950.052.0321367