FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286141
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1078734.718562853297325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281651.2319887530996558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG66020.28878358771396867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG37230.16285084778235462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG36970.16171356009974888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG30750.13450613938510353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA87450.067.91151454
GGGGTTA87300.067.3051553
GGTTAAA88850.067.03985655
GGGGGTT96600.064.6321652
GTTAAAA95650.063.7007856
TTGGGGG164550.062.7641739
TTTGGGG165050.062.42696838
TGGGGGG177650.059.67384340
AGGGGGG199250.059.0351570
GGGGGGT115200.058.53715551
GGGGCCG19400.058.08782245
TTTTGGG177150.057.35170737
GGGCCGG19600.057.1354746
GGCCGGG19900.055.3948547
AAGGGGG217250.053.93325469
AAAGGGG219250.053.17923768
GCCGGGG20850.052.36847748
AAAAGGG226600.051.40909667
TTTTTGG201350.050.29893536
AAAAAGG239300.048.7977666