FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140539
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG976104.56006641317911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250971.1724617024029929No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG59470.27782722015342864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG41220.19256832040901847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG36130.16878926289126242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG24030.112261444430585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA74100.067.0708354
GGGGTTA73600.066.8131653
GGTTAAA75850.065.8094955
GGGGGTT81750.064.34344552
TTGGGGG145950.063.54701639
TTTGGGG145050.063.30019838
GTTAAAA84550.061.06648656
TGGGGGG160700.059.4783940
AGGGGGG176800.058.68684470
GGGGGGT99300.057.0977751
TTTTGGG159550.056.8440437
GGCCGGG17250.055.8004447
GGGGCCG17950.054.98678645
GGGCCGG18050.054.48824746
GCCGGGG17550.054.25333848
AAGGGGG193050.053.45424369
AAAGGGG195350.052.75691668
AAAAGGG203350.050.59414367
TTTTTGG185800.048.8427436
AAAAAGG213950.048.15406866