FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453286
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1061387.3033112546326056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258371.777833131262532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG74700.5140075663014713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG45120.31046882719574814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG39110.2691142693179457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG25810.1775975272589153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA77750.068.3848154
GGGGTTA77100.068.141853
GGTTAAA78550.067.908855
GGGGGTT85000.066.0042652
TTGGGGG152950.064.9640739
GTTAAAA85050.064.73537456
TTTGGGG152850.064.4134638
TGGGGGG167500.060.90894740
TTTTGGG160150.060.42831437
GGGGGGT104000.057.95049751
GCCGGGG16800.057.92322548
AGGGGGG195500.057.92215770
GGGGCCG17300.057.6615145
GGGCCGG17450.057.3644646
GGCCGGG17150.057.14341747
CCGGGGA12000.056.00441449
TTTTTGG176950.054.3888136
AAGGGGG213350.052.89742769
AAAGGGG212600.052.86081368
AAAAGGG216550.051.86247667