FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990137
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1614488.112406331825397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397611.9979026569527627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG112090.5632275566958456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC97730.49107172018810763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG58090.291889452836664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG55590.2793275035839241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG42870.21541230578598358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA124550.068.7057154
GGGGTTA123850.068.5571153
GGTTAAA126000.068.3663755
GGGGGTT136200.066.0667152
GTTAAAA132800.066.05176556
TTGGGGG238750.065.32201439
TTTGGGG237400.065.1037638
TGGGGGG257250.061.9849340
TTTTGGG247700.061.6461637
GGGGGGT160950.060.1274651
GGGGCCG24150.059.7117345
GGGCCGG24700.058.8087146
AGGGGGG295100.058.48953670
GGCCGGG24400.057.95236247
TTTTTGG268750.056.6947836
GCCGGGG24950.055.69566748
CCGGGGA16500.054.09098449
AAGGGGG321500.053.39135769
AAAGGGG322100.053.2782168
AAAAGGG327600.052.33162367