FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886034
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG27672014.672057873824121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG643733.4131410144249785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG191871.0173199422703938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC171070.907035610174578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG91910.4873188924483864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG88740.470511136066476No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG84340.4471817581231303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG29310.15540546989078668No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG28180.14941406146442746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA213250.069.5497654
GGGGTTA211850.069.3339253
GGTTAAA215700.069.3114355
GGGGGTT228200.068.42860452
GTTAAAA224750.067.9081156
TTTGGGG394150.066.199638
TTGGGGG399950.065.65620439
TTTTGGG397700.064.7003837
GGCCGGG39700.063.02883547
CCGGGGA25700.062.9118749
GGGCCGG40550.062.8262646
GCCGGGG39950.062.1170548
TTTTTGG415150.061.91708436
TGGGGGG434750.061.80161740
GGGGGGT269700.061.54217551
GGGGCCG42250.061.37509545
GGGCCCC8350.059.9228749
AGGGGGG492900.059.73561570
GGGTTTA15750.059.5494854
GGTTTAA15800.059.36103455