FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452646
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1911267.79264516770867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG463231.8886949033818985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG131260.5351771107611942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG63900.2605349487859235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG61800.2519727673704236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG59170.24124965445482144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA149250.068.6853454
GGGGTTA148450.068.443953
GGTTAAA151600.067.9941855
GGGGGTT163300.066.2277852
GTTAAAA159700.065.8605356
TTTGGGG282300.064.41498638
TTGGGGG284250.064.34563439
TTTTGGG293950.061.2773137
TGGGGGG305950.061.08590340
GGGGCCG30550.060.49098245
GGGGGGT194050.060.0966751
GGGCCGG30550.059.91692746
AGGGGGG348550.059.40211570
GGCCGGG30900.057.88260347
GCCGGGG31000.057.13601348
TTTTTGG322200.055.82286536
CCGGGGA20800.055.19229549
AAGGGGG379800.054.29808869
AAAGGGG380150.054.13503668
AAAAGGG388200.052.931367