FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451779
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1317205.372425491857137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320151.3057865329623919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG91900.3748298684343083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC77600.31650487258435606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG58180.2372970810174979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG48570.19810105233791464No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG33290.1357789588702734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA102550.068.28512654
GGGGTTA102150.067.97288553
GGTTAAA104200.067.6068655
GGGGGTT114200.064.87646552
TTGGGGG200750.063.8010839
GTTAAAA115250.063.12890256
TTTGGGG201350.063.1280538
TGGGGGG217750.060.6217440
GGGGGGT133750.059.0592551
GGGGCCG23300.058.58487345
TTTTGGG215850.058.17256537
AGGGGGG250300.057.81941670
GGGCCGG23950.056.4091746
GGCCGGG23650.056.2367747
GCCGGGG23650.054.90933248
AAGGGGG272500.052.96446669
AAAGGGG274900.052.33131468
TTTTTGG243200.051.6508936
AAAAGGG283400.050.661967
GGGGGCC27300.049.99998544