FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231667
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1771587.938370733626478No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434171.9454963486936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG106420.4768632596171382No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG82910.3715160012672141No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG69680.31223296307199955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG41200.18461535704027526No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG22850.1023898278730653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA141000.068.8014754
GGGGTTA140200.068.4951353
GGTTAAA142600.068.3899855
GGGGGTT153550.066.09452
GTTAAAA152600.065.8840456
TTTGGGG265850.065.1865338
TTGGGGG268450.064.8492639
TTTTGGG273650.062.29240837
TGGGGGG293050.061.2925340
GGGGGGT180800.060.3533451
GGGCCGG30250.059.9264146
GGGGCCG30350.059.84829345
GGCCGGG30050.058.93172547
AGGGGGG332500.058.7177170
GCCGGGG30350.057.77781348
TTTTTGG297700.057.10079636
CCGGGGA21050.055.19749549
AAAGGGG362600.053.51516368
AAGGGGG363700.053.46042669
AAAAGGG369750.052.42708667