FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476911
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG15361310.400965257892993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374922.5385415911994698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG92960.6294218135012875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC92000.6229217603498112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG77990.528061609670454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG61510.4164773639034444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG33950.22987167134647926No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG20350.13778758503389846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG16460.11144882799302055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA122350.069.3882454
GGGGTTA122650.068.75967453
GGTTAAA124900.068.6346255
GGGGGTT132650.067.2957352
GTTAAAA132600.066.734556
TTTGGGG226100.065.8610238
TTGGGGG227950.065.6948339
TTTTGGG231100.063.0252637
TGGGGGG251350.061.86018440
GGGGGGT156950.060.91211351
GGCCGGG24350.060.37434847
TTTTTGG242700.059.907936
GGGCCGG25150.059.83956546
CCGGGGA17350.059.3009449
GGGGCCG25350.059.23140345
GCCGGGG24800.058.57711848
AGGGGGG288600.058.5701370
GGGCCCC5450.055.8551069
TATGGGG8750.054.80801838
GGGGCCC5450.054.572938