FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943858
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1813889.33134004644372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448042.304900872388827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG115050.5918642205346275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC111250.5723154674878514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG73140.37626205206347374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG65460.33675299327420005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG46950.24152998830161465No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21120.10864991167050268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA142700.068.8946454
GGGGTTA141950.068.56827553
GGTTAAA144650.068.3134255
GGGGGTT155000.066.585352
GTTAAAA154350.065.78921556
TTTGGGG262000.065.1108138
TTGGGGG264050.065.0294739
TTTTGGG269750.062.20215237
TGGGGGG289150.061.1259840
GGGGGGT184800.060.03025451
AGGGGGG331350.058.80353570
CCGGGGA18150.058.61967549
TTTTTGG290350.057.67603336
GGGGCCG29300.057.57393345
GGCCGGG28200.056.83968447
GGGCCGG29200.056.68800446
GCCGGGG28200.056.3461748
AAAGGGG358100.054.0101268
AAGGGGG359350.054.0073469
GGGTTTA11750.053.61573854