FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649833
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG910213.4349711849765625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231610.874055081961769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG55600.2098245436599212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG41350.15604756979024717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG35500.13397070683322307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA72150.066.88988554
GGGGTTA72500.066.0372353
GGTTAAA73400.065.9439655
GGGGGTT80950.063.29435352
TTGGGGG142500.062.39029339
TTTGGGG145450.060.78914638
GTTAAAA82000.060.5621256
TGGGGGG154750.059.48829340
GGGGGGT95450.057.6035451
AGGGGGG177500.056.77617670
TTTTGGG164100.053.11054237
GGGGCCG17600.052.70152745
AAGGGGG190850.052.50624569
AAAGGGG192200.051.9641868
GGCCGGG17150.051.42922647
GGGCCGG18050.050.8039746
GCCGGGG16700.050.3019448
AAAAGGG200950.049.54189367
AAAAAGG215950.046.1979566
GGGGGCC20000.045.32814444