FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007343
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG942424.69486281118872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247161.231279357837699No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG59930.29855385950482805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC55600.27698305670729917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG43850.21844796828444368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG37710.18786027101496855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG21720.10820273366335499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA74550.068.26626654
GGGGTTA74350.067.88328653
GGTTAAA75400.067.728855
GGGGGTT83250.064.1092752
TTGGGGG147100.063.0062939
TTTGGGG146500.062.7943438
GTTAAAA84500.062.2559556
TGGGGGG160600.059.4505340
TTTTGGG157000.057.54535737
AGGGGGG183750.056.92986770
GGGGGGT101300.056.34956751
GGGGCCG17250.056.2000545
GGGCCGG17100.056.07903346
GGCCGGG16700.055.74846347
GCCGGGG16950.054.30945648
AAGGGGG197700.052.60783469
AAAGGGG198000.052.44757568
AAAAGGG204000.050.8166567
TTTTTGG182700.049.57603536
AAAAAGG216550.048.14645466