FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152538
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1169115.431309458880633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292661.3596043368340072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG81720.37964486573523903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC64420.2992746237232513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG58680.2726084278186959No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG48650.2260122701666591No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG26170.12157741233836522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA90050.068.05862454
GGGGTTA89450.067.8890853
GGTTAAA91900.066.8789955
GGGGGTT97450.065.2205452
TTGGGGG175950.064.6310439
TTTGGGG176050.063.93974338
GTTAAAA100250.063.40335556
TGGGGGG195100.060.76292440
GGGCCGG19550.058.8992446
GGGGGGT119300.058.2652351
TTTTGGG189800.058.0905137
GGGGCCG20800.057.21055245
AGGGGGG223900.057.1977570
GGCCGGG20000.056.0016447
GCCGGGG20250.054.27697848
AAGGGGG239350.053.35817769
AAAGGGG241350.052.7614168
TTTTTGG210750.052.27783236
AAAAGGG249000.051.00103467
CCGGGGA16000.049.00029449